Table 2.
Kinetic parameters of Aβ(4–10)Q (Abz‐AβFRHDSG(Q)‐EDDnp) hydrolysis by different ACE variants/molecules. Error is represented as standard error of the mean (±SEM) barring the k cat/K m values where the error represents the fractional error of the ratio; n = 3. ND, not determined
| Enzyme | k cat/K m (m −1·s−1 × 105) | k cat (s−1) | K m (μm) | V max | E (pmol) |
|---|---|---|---|---|---|
| Ndom | 0.22 ± 0.04 | 0.48 ± 0.04 | 22.20 ± 3.23 | 0.29 ± 0.02 | 0.60 |
| N‐sACE | 0.09 ± 0.01 | 0.36 ± 0.02 | 42.43 ± 3.92 | 3.23 ± 0.20 | 9.00 |
| sACE | 0.03 ± 0.01 | 0.06 ± 0.01 | 22.90 ± 3.45 | 0.09 ± 0.01 | 1.50 |
| C‐sACE | 0.08 ± 0.02 | 0.08 ± 0.01 | 11.50 ± 3.03 | 0.12 ± 0.01 | 1.50 |
| CC‐sACE | ND | ND | ND | ND | ND |
| Cdom | ND | ND | ND | ND | ND |