Table 3.
Kinetic parameters of Aβ(4–10)Y (Abz‐FRHDSG) hydrolysis by different ACE variants/molecules. Error is represented as standard error of the mean (±SEM) barring the k cat/K m values where the error represents the fractional error of the ratio; n = 3
| Enzyme | k cat/K m (m −1·s−1 × 105) | k cat (s−1) | K m (μm) | V max | E (pmol) |
|---|---|---|---|---|---|
| Ndom | 9.57 ± 0.33 | 20.30 ± 0.62 | 21.20 ± 0.31 | 4.62 ± 0.14 | 0.228 |
| N‐sACE | 4.61 ± 0.46 | 3.87 ± 0.09 | 8.56 ± 0.83 | 0.44 ± 0.01 | 0.113 |
| sACE | 22.67 ± 6.40 | 28.70 ± 5.81 | 12.60 ± 2.48 | 0.90 ± 0.18 | 0.031 |
| C‐sACE | 1.83 ± 0.18 | 6.95 ± 1.39 | 38.10 ± 0.10 | 1.57 ± 0.09 | 0.226 |
| CC‐sACE | 6.33 ± 0.98 | 12.70 ± 1.16 | 20.30 ± 2.55 | 0.90 ± 0.08 | 0.071 |
| Cdom | 2.30 ± 0.87 | 5.33 ± 0.76 | 27.23 ± 9.52 | 4.45 ± 0.63 | 0.835 |