Table 5.
Aβ cleavage products from prolonged ACE hydrolysis. The observed cleavage product [M + H]+ ions generated by the various ACE constructs on the Aβ(1–16) substrate over a 24‐h period. ND, not determined
| Amyloid peptide | Peptide residues | Calculated m/z | Observed m/z | |||||
|---|---|---|---|---|---|---|---|---|
| Ndom | N‐sACE | sACE | C‐sACE | CC‐sACE | Cdom | |||
| Aβ(1–16) | DAEFRHDSGYEVHHQK | 1954.8 | 1954.8 | 1954.8 | 1954.8 | 1954.8 | 1954.8 | 1954.8 |
| N‐terminal products | ||||||||
| Aβ(1–14) | DAEFRHDSGYEVHH | 1698.7 | 1698.7 | ND | 1698.7 | 1698.7 | 1698.6 | 1698.7 |
| Aβ(1–12) | DAEFRHDSGYEV | 1424.6 | 1424.6 | 1424.6 | 1424.6 | 1424.6 | 1424.6 | 1424.6 |
| Aβ(1–11) | DAEFRHDSGYE | 1325.5 | ND | 1324.5 | ND | 1325.5 | 1325.5 | ND |
| Aβ(1–7) | DAEFRHD | 889.3 | 889.3 | ND | 889.3 | 889.3 | ND | 889.3 |
| Aβ(1–6) | DAEFRH | 774.4 | ND | ND | ND | 774.4 | ND | ND |
| Aβ(1–5) | DAEFR | 637.3 | 637.3 | ND | 637.3 | 637.3 | 637.3 | 637.3 |
| C‐terminal products | ||||||||
| Aβ(2–16) | AEFRHDSGYEVHHQK | 1839.8 | ND | ND | ND | ND | ND | 1839.8 |
| Aβ(4–16) | FRHDSGYEVHHQK | 1639.7 | 1639.7 | ND | ND | ND | ND | ND |
| Aβ(12–16) | VHHQK | 648.3 | 648.3 | ND | 648.3 | 648.3 | ND | 648.3 |