Figure 3.

Effects of PAR-2 activating peptide on eNOS phosphorylation and NO production in HCAECs (a) HCAECs were stimulated with SLIGRL (50 µmol/L) for the indicated time points and phosphorylated eNOS-ser1177 and eNOS-Thr495 was determined using Western blot analysis with phosphospecific antibodies. Representative immunoblots are shown with densitometric analyses. Mean +/− SEM of measurements in cultures of six different passages; ***P < 0 .001 SLIGRL Thr495p 10 min versus Basal, *P < 0.05 SLIGRL 15 min and 30 min versus Basal, *P < 0.05 SLIGRL Ser1177p 0.5 min and 2 min versus Basal. (b) NO production was determined indirectly by measuring total nitrate/nitrite using media from HCAECs treated with SLIGRL (50 µmol/L) for the indicated time points, n = 6, NS