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. 2016 Jan 31;241(6):611–619. doi: 10.1177/1535370216629011

Figure 3.

Figure 3

Proline increased ER-β mRNA expression and estrogenic activity in MG63 and MCF-7 cells. (a) MG63 and MCF-7 cells were treated with proline (10 µg/ml) or 17β-estradiol (100 nM) for 10 h. The ER-β mRNA expression was analyzed with the quantitative real-time PCR analysis. (b) MG63 and MCF-7 cells were treated with proline (10 µg/ml) or 17β-estradiol (100 nM) for 48 h. The ERE-luc activity was measured with a luciferase assay. *P < 0.05; significantly different from untreated cells. Blank, untreated cells