Figure 5.

The functional beneficial effects of the myosin transgene do not involve the PI3K‐Akt‐mTOR signaling in transgenic mice. This figure shows typical Western blots of the tibialis anterior muscles from transgenic mice. These muscles were injected with ∼1 × 10¹⁰ vector genomes of rAAV6 containing either the MYL4 gene (referred to as MYL4) or a vector lacking a functional gene (referred to as MCS). The p‐Akt to Akt and p‐mTOR to mTOR ratios were calculated and represented the Akt and mTOR activities, respectively. Values in the graphs are mean ± standard error of the mean. [Color figure can be viewed in the online issue, which is available at www.annalsofneurology.org.]