Figure 5.

Effect of ryanodine on constant-voltage currents under SOCE conditions. Calcium was depleted by thapsigargin incubation (see Materials and methods section). The chelator BAPTA was also included in the internal pipet solution to ensure depletion of calcium. The holding potential was −80 mV and voltage ramps conducted from −70 mV to +60 mV for 450 ms at 1 s intervals. Ryanodine was present at zero (circles) or 10 μM (squares) concentrations in the external solution. No significant ryanodine inhibition was observed at P < 0.05, n = 6