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. 2016 Jul 19;11:100. doi: 10.1186/s13023-016-0481-4

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Outline of methodological approach. In the first step, rough brain masks were manually created for cerebellum, brain stem, supratentorial brain volume and frontal lobe. Following this, image intensity threshold was automatically applied and manually adjusted, in order to remove CSF signal within the brain mask