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. Author manuscript; available in PMC: 2016 Jul 19.
Published in final edited form as: J Am Chem Soc. 2016 Mar 23;138(13):4439–4447. doi: 10.1021/jacs.5b13107

Figure 1.

Figure 1

Manufacture of uniform-sized DNA-origami templated SUV that bears pre-determined numbers of VAMP2 (v-SNAREs) and v-tethers. Illustrated is an example of a DNA origami-templated vesicle, where the template displays 4 full length VAMP2 (aa 1–116), 12 ssDNA-lipid anchors. A number of v-tethers, not attached to the template, diffuse freely on the external surface of a vesicle. The detailed preparation procedure is described in the Material and Methods section in the Supplementary Information. Briefly, (f) was first prepared by mixing and incubating individually purified components (c), (d), and (e). After purification, complex (f) was reconstituted into (g) and the final product (h) was obtained. Anti-handles holding DOPE anchors are not shown in (h) cross-section view for clarity. Color codes: Grey annulus, DNA origami-ring containing a total of 16 inner handles, with 4 of “handle i” to direct VAMP2 and 12 of “handle ii” to direct DOPE lipid anchors; Green, full-length VAMP2; Orange, DOPE lipid anchors; Red in different shades, ssDNA with different sequences on templated-SUV, serving as anti-handles and v-tethers (see Table S1 for oligonucleotide sequences).