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. Author manuscript; available in PMC: 2016 Jul 19.
Published in final edited form as: J Am Chem Soc. 2016 Mar 23;138(13):4439–4447. doi: 10.1021/jacs.5b13107

Figure 3.

Figure 3

Single-molecule step-bleaching experiments confirming the number of SNAREs on each vesicle. (A) Selected fluorescent spots (particles in blue circles) for analysis of 4×VAMP2 ring (for an area of 91 μm × 91 μm). (B) Examples of mean fluorescence intensity (area of 6×6 pixels) time courses showing bleaching steps. (C) – (F) Distribution of bleaching steps of rings with different numbers of labeled VAMP2. The observed distributions match calculated distributions assuming 75% yield of binding of VAMP2 to each handle and 10% probability of two spots co-localized with one another during the measurements.