Figure 2.

ZGDHu-1 modulates cell cycle-related protein levels in A549 and RERF-LC-MA cells. Western blot analysis of G₂/M cell cycle control proteins (cyclin B1, Cdc2, Cdc25c and p53) levels in (A) A549 and (B) RERF-LC-MA cells. Quantification of (C) cdc2, (D) cdc25c,(E) cyclinB1 and (F) P53, respectively. *P<0.05, compared with control The cells were treated with various concentrations of ZGDHU-1 (0, 100, 200 and 500 µg/l) for 48 h. β-actin was used as a loading control. ZGDHu-1, N, N′-di-(m-methylphenyi)-3,6-dimethyl-1,4-dihydro-1,2,4, 5-tetrazine-1,4-dicarboamide; M, mitotic; Cdc25c, cell division cycle 25c.