Figure 5.

Regulation of Bcl-2 expression by TIIA. (A and B) H9c2 cells were incubated with400 µM H₂O₂ or 3 µM TIIA, then harvested and lysed. A western blot was performed to examine Bcl-2 protein expression. β-actin was used as a protein loading control. (A) A reduction in Bcl-2 expression was observed in response to 400 µM H₂O₂ treatment, while (B) TIIA increased Bcl-2 expression in a time-dependent manner. (C) H9c2 cells were pre-incubated with wortmannin (100 nM) for 30 min then treated with 400 µM H₂O₂ in the presence or absence of 3 µM TIIA for 24 h. Wortmannin treatment decreased the Bcl-2 protein expression induced by TIIA. TIIA, tanshinone IIA; Bcl-2, B cell lymphoma-2.