Figure 1.

Lentiviral transfection and analysis of cell proliferation. (A) Diagram of the EZH2 overexpression lentiviral vector system. (B) Western blotting was used to determine the degree of EZH2 overexpression in the AMC-HN-8 cell lines. (C) Reverse transcription-quantitative polymerase chain reaction was used to determine the degree of EZH2 overexpression at the messenger RNA level in the AMC-HN-8 cell lines. (D) The proliferation assay was performed on the AMC-HN-8 cells and EZH2-OE cells on days 1, 3, 5 and 7 of culture using the cell counting kit-8 method. The results were expressed as the mean values ± standard deviation. *P<0.05. EZH2, enhancer of zeste 2 polycomb repressive complex 2 subunit; EZH2-OE, EZH2-overexpressing.