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Direct in vitro capture of embryo-derived epiblast cells. Single ICM cells were isolated from pre- and post-implantation embryos, and either pooled and expression profiled (not shown), or plated in medium containing 2i-LIF (see text for details) for the derivation of self-renewing naive-state ESCs. Arrows indicate the capacity of individual embryo-derived cells to give rise to ESCs.
