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Representative measurements of AFP methylation using pyrosequencing. (A) AFP locus cg10778295 methylation status in cancer tissues (79% methylation). (B) AFP locus cg10778295 methylation status in normal tissues (91% methylation). The methylation percentages are percentages of C at each CpG site after bisulfite conversion. Orange bar indicates no residual C at the non-CpG site, ensuring complete bisulfite conversion. AFP, alpha-fetoprotein.
