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. 2016 Jul 19;17:85. doi: 10.1186/s12931-016-0407-7

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Silver-enhancing and hematoxylin-eosin-stained Sprague–Dawley (SD) and Brown-Norway (BN) rat lung sections at 24 h post-treatment. Panels a & d: SD and BN air control shows an absence of signal; silver-stained positive cells are seen as black or black brown positivity (red arrows). In Panel b, SD at lung dose of 8 μg; in Panel c, at lung dose of 28 μg AgNPs and in Panel e, BN at lung dose 8 μg and Panel f, 26 μg AgNPs. Positive silver-stained cells are deposited in granuloma of BN lungs (arrow head pointing multi-nuclear giant cells). There are high levels of eosinophilic inflammation (black arrows) in BN lungs (internal scale bar = 20 μm for all)