Table 2.

Representative examples of Fungal CYP expression and their kinetic parameters

CYP	CYP source	Expression host	Expression level	Substrate	CPR source	Kinetic parameters	Notes	Ref
CYP53A1	A. niger	A. niger	160 pmol/mg of protein (Microsomal fraction)	Benzoic acid	A. niger	k cat; 270 ± 30 nmol min−1 nmol P450−1, K m; 0.083 ± 0.009 mM	Para-hydroxylation	[94]
CYP65A2	P. chrysosporium	S. cerevisiae	1 nmol/L	Naringenin	S. cerevisiae	k cat; 0.29 ± 0.02 min−1, K m; 391 ± 27 μM	3′-Hydroxylation	[48]
CYP50C	P. chrysosporium	S. cerevisiae	40 pmol/mg of protein (Microsomal fraction)	Flavone	S. cerevisiae	k cat; 0.52 ± 0.03 min−1, n; 2.2 ± 0.1, K S; 109 ± 6 µM	3′-Hydroxylation, Hill equation was used for kinetic parameters	[49]
CYP142C	P. chrysosporium	S. cerevisiae	119 pmol/mg of protein (Microsomal fraction)	Flavone	S. cerevisiae	k cat; 0.019 ± 0.002 min−1, n; 2.0 ± 0.4, K S; 152 ± 30 µM	3′-Hydroxylation, Hill equation was used for kinetic parameters	[49]
CYP52M1	S. bombicola	S. cerevisiae	NA	Oleic acid	Arabidopsis thaliana	k cat; 535 ± 60 pmol min−1 mg P450−1, K m; 40 ± 2 µM	ω-Hydroxylation	[44]
PcCYP1f	P. chrysosporium	P. pastoris	NA	Benzoic acid	P. chrysosporium	k cat; 0.013 μmol min−1μmol P450−1, K m; 185 µM	Para-hydroxylation	[39]
CYP5150A2	P. chrysosporium	E. coli	200–300 nmol/L	4-Propylbenzoic acid	P. chrysosporium	Initial velocity (μmol/min/μmol of P450); 0.3 ± 0.1 with CPR, 0.6 ± 0.1 with CPR, Cyt b5 and 10.6 ± 0.3 with CB5R, Cyt b5	Formation of 4-(2-hydroxypropyl)benzoic acid, ∆ 13 form of CYP5150A2 coexpressed with GroEL/ES. CB5R/Cyt b5 act as electron partner in the absence of CPR	[31]
MgCYP51	M. graminicola	E. coli	NA	Eburicol	M. graminicola	k cat; 0.13 min−1, K m; 33 µM	14α- demethylation, Sterol selective and Temperature sensitive enzyme	[33]
CYP53A15	C. lunatus	E. coli	800–900 nmol/L	Benzoic acid	Mammal	k cat; 1.4 ± 0.5 min−1, K m; 1.4 ± 0.2 mM	para-hydroxylation	[37]
CYP52A21	C. albicans	E. coli	60 nmol/L	Dodecanoic acid	Rat	k cat; 33 ± 1 min−1, K m; 57 ± 2 µM	ω-Hydroxylation	[74]
CYP630B18	G. clavigera	E. coli	3.1 μmol/L	Oleic acid	G. clavigera	NA	ω-Hydroxylation	[47]
304 CYP isoforms	P. chrysosporium and P. placenta	E. coli	43–1255 nmol/L	NA	–	NA	27 CYPs were expressed in active form with/without NTD modifications among 304 CYPs	[75]

NA information not available