Figure 1.

(A) Effects of TRPV4 antagonists (20 μM RN1734, 10 μM HC067047 and 10 μM ruthenium red) and endothelial removal on GSK‐induced relaxation in mesenteric arteries. The vehicle (and time) controls for GSK (0.001–0.03% vv⁻¹ DMSO, n = 5) are also shown. Values are shown as means and vertical bars represent SEM. The data were analysed by two‐way ANOVA, followed by Bonferroni post hoc test. ** P < 0.01 in comparison with control (+ec); (B) An original trace showing the relaxant response to GSK in an endothelium‐intact mesenteric artery precontracted with 10 μM methoxamine (methox). The arrows indicate where the maximal relaxant effect was determined at each concentration.