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. 2016 Jun 8;12(2):977–982. doi: 10.3892/ol.2016.4685

Figure 2.

Figure 2.

(A) Effect of transfection of human vestibular schwannoma HEI-193 cells. Lane 1, transfected HEI-193 cells with plasmid encoding AS NF2. Lane 2, transfected HEI-193 cells with plasmid encoding WT NF2. (B) Levels of NF2 protein in HSCs: The AS isoform was highly expressed in HSCs at 38 kDa, while the WT isoform, which was expected at 66 kDa, was undetectable. (C) The novel AS NF2 isoform had no suppressive effect on cell proliferation. HEI-193 cells were transfected with recombinant plasmids expressing WT, AS NF2 or the two isoforms. The effect of the expression of these isoforms on cell proliferation was measured using a cell counting kit-8 assay for 3 days. M, DNA marker DL2000; AS, alternative splicing; WT, wild-type; HSC, human Schwannoma cells; Ctrl, control; A450, absorbance at 450 nm; IP, immunoprecipitation.