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. 2016 Jun 8;12(2):1058–1066. doi: 10.3892/ol.2016.4686

Figure 5.

Figure 5.

Effects of HP treatment and inhibition of the MAPK signaling pathway on the expression of AKT and mitochondrial pathway proteins. (A) Cells were treated with HP (100 µg/ml) for 0, 4, 8, 12, 24 and 48 h. The different expression of the B-cell lymphoma-2 family proteins indicated that the mitochondrial pathway was affected after the treatment with HP. The expression of GAPDH was used as an internal control. All immunoblotting experiments were performed twice. (B and C) Effects of inhibiting the MAPK signaling pathway on the mitochondrial pathway proteins in (B) HepG2 and (C) Huh7 cells. HP treatment at a concentration of 100 µg/ml for 24 h, with or without MAPK inhibitors (PD98059, 20 µM; SP600125, 10 µM; and SB203580, 10 µM). The statistical results are presented in a column diagram. Data are represented as the mean ± standard error of the mean from three independent experiments. *P<0.05 and **P<0.01 indicate a significant alteration in the expression levels of proteins after the treatment with MAPK inhibitors. The expression of GAPDH was used as an internal control. All immunoblotting experiments were performed in duplicates. Bcl, B-cell lymphoma; Bax, Bcl-2-associated X protein; xL, extra large; Mcl-1, myeloid cell leukemia-1; Bim, Bcl-2-like 11; HP, Huaier polysaccharide; p, phosphorylated; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; MAPK, mitogen-activated protein kinase.