T cell Ig and mucin domain-containing molecule-3 (TIM-3) expression in adult T-cell leukemia/lymphoma (ATLL) cell lines. (A) Outline of the ATLL/macrophage co-culture methods used and flow cytometric analysis of the cells. (B) TIM-3 expression in the CD14-negative ATLL cell lines (ATN-1, TL-Mor, ED and ATL-2S) was evaluated pre- and post-co-culture with macrophages, using flow cytometry. (C) The percentage of TIM-3-positive ATN-1 cells was evaluated after the indicated weeks (w) of co-culture with macrophages. SSC, sidewards scatter; CD, cluster of differentiation.