Table I.

Sequences of the primers used for the eight exons of the RUNX2 gene.

Exon	Primer sequences (3′-5′)	Annealing temperature °C
0	F: ATGGTTAATCTCCGCAGGTCA	58.0
	R: GCTATTTGGAAAAGCTAGCAG
1	F: CCAAAGACTCCGGCAAAGAT	56.5
	R: AAGGCAGGAGGTCTTGGAG
2	F: TGGCATCACAACCCATACAC	59.0
	R: GTCTACATTTCATCAAAGGAGC
3	F: AATTTAGAAGAAGGAGTCCTG	59.0
	R: AAATATATGCAGATAGCAAAG
4	F: ATTCCTTGGCTTAAACTCCCAG	56.0
	R: GCCAGCTTCACAGCTCCAGG
5	F: AACGCTTTGTGCTATTTAAGGCC	61.0
	R: CCAGTTGTCATTCCCTTGCCC
6	F: CTCTGGGAAATACTAATGAGGGA	61.0
	R: AGTGCCATGATGTGCATTTGTAAT
7	F: GGCTTGCTGTTCCTTTATGG	60.0
	R: GGCTGCAAGATCATGACTGA

RUNX2, runt-related transcription factor 2; F, forward; R, reverse.