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. 2016 May 23;12(2):711–719. doi: 10.3892/etm.2016.3380

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — Type II collagen immunohistochemical staining. (A) In the chondroblast-induced group, the cells displayed brownish yellow granules in the cytoplasm following type II collagen immunohistochemical staining (magnification, ×200). (B) In the osteoblast-induced group (magnification, ×200), gray-black granules or black deposit were observed in the (C) cytoplasm (magnification, ×200) and (D) Alizarin red staining revealed a large number of mineral nodes in the osteoblast-induced group (magnification, ×400).