Table 2.
ChromID VRE direct plating Method 1 |
Brilliance VRE direct plating Method 2 |
ChromID VRE plating after enrichment Method 3 |
Brilliance VRE plating after enrichment Method 4 |
|||||
---|---|---|---|---|---|---|---|---|
Incubation time (hours) | 24 | 48 | 24 | 48 | 24 | 48 | 24 | 48 |
Number of plates growing VREa | 10 | 28 | 14 | 29 | 32 | 33 | 33 | 34 |
Sensitivityb | 29% | 82% | 41% | 85% | 94% | 97% | 97% | 100% |
Number of plates NOT growing organisms other than VREa | 292 | 277 | 293 | 285 | 259 | 227 | 280 | 270 |
Selectivityc | 99% | 94% | 99% | 97% | 88% | 77% | 95% | 92% |
Number of plates growing Gram-negative organisms | 3 | 10 | 2 | 5 | 28 | 58 | 11 | 18 |
Number of plates growing Gram-positive organisms | 0 | 6 | 0 | 4 | 6 | 7 | 4 | 6 |
Number of plates growing yeasts | 0 | 2 | 0 | 1 | 2 | 3 | 0 | 1 |
VRE = vancomycin-resistant enterococci.
Target VRE organisms consisted of vancomycin-resistant E. faecalis (VREfs) and vancomycin-resistant E. faecium (VREfm).
Sensitivity was calculated by comparing the number of positive plates for a given method, compared with the cumulative yield of VREfs or VREfm from all four methods (direct plating onto two media, and plating onto both following a pre-enrichment step, n = 34).
Selectivity for each method was defined as the number of plates that suppressed the growth of organisms that were not VREfs or VREfm (out of all 295 samples).