Fig. 11.

Comparison of phenotypic characteristics of BMSC grown on LDI–glycerol polymer and TCPS: (a) total hydroxyproline contents as a measure of synthesis and secretion of collagen type I in BMSC grown on LDI–glycerol polymeric foam and TCPS; (b) comparison of BMSC proliferation on LDI–glycerol and TCPS. BMSCs were grown on TCPS or LDI–glycerol poly(urea–urethane) for 7 or 14 days, were trypsinized and viable cells counted by trypan blue exclusion. Non-viable cells were less than 1% in each case; (c) comparison of mRNA expression for collagen type I (COL I) and TGF-β1 in BMSC cultured on LDI–glycerol polymer or TCPS for 7 or 14 days. Expression of mRNA for glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a house keeping gene, was used as a control to show equivalent input of RNA in all lanes.