Fig 1. Developmental gene expression in individual human blastocysts (n = 10 replicates for each group) was performed by qPCR.

Ct values were normalized to PPIA, an internal, constant housekeeping gene. Fold change was determined using the ΔΔCt method on the average of technical duplicates. Error bars represent standard error and the y-axis denotes fold change between euploid and aneuploidy. A) Signigicant decreased expression of chromosome 15 genes in monosomy 15 blastocysts compared to controls (*P < 0.05). B) Significant decreased expression of chromosome 22 genes in monosomy 22 blastocysts compared to controls (*P < 0.05).