Table 1. Primer information and qPCR efficiencies for chromosomes 15, 22, and housekeeping genes.
| Gene | Accession # | Slope | Amplicon GC | Chromosome | Primer Sequence (3'-5') |
|---|---|---|---|---|---|
| Intercept | Amplicon Length | ||||
| R2 | |||||
| AVEN | NM_020371 | -3.29 | 46% | 15 | F: AAGAGCTGGAAGACTGGTTGGA |
| 28.37 | 98 | R: TATGCCCACCTGCCGTTAG | |||
| 0.99 | |||||
| CYFIP1 | NM_014608 | -3.51 | 51% | 15 | F: ACGACCACTCAGCGTACAAGAG |
| 25.71 | 78 | R: TCTGCGATTCCTGGATGGA | |||
| 0.99 | |||||
| FAM189A1 | NM_015307 | -3.07 | 62% | 15 | F: GGGACACCCAGGATGATCTG |
| 31.47 | 97 | R: GGAAATGCAATCCCCAAAGAG | |||
| 0.99 | |||||
| MYO9A | NM_006901 | -3.26 | 45% | 15 | F: CAATACACTGGAACGCCTCATC |
| 27.11 | 92 | R: ACACAATGGCCAAAGCATTAGC | |||
| 0.99 | |||||
| ADM2 | NM_001253845 | -2.97 | 59% | 22 | F: GAGCCTAAACACCCTGAAATTGTG |
| 28.92 | 88 | R: TCTCTGAAGCGCTTAGCATCTG | |||
| 0.99 | |||||
| PACSIN2 | NM_001184970 | -3.57 | 54% | 22 | F: AAGCCCTGGGCCAAGAAG |
| 26.99 | 59 | R: GCTGCATGGTGGGCTTTC | |||
| 0.99 | |||||
| PARVB | NM_001003828 | -3.45 | 62% | 22 | F: TCTCTGGCCATGCACTTCAG |
| 25.31 | 65 | R: ACCACCACCTGCACCGTTAC | |||
| 0.99 | |||||
| PIWIL3 | NM_001008496 | -1.51 | 44% | 22 | F: AAAGAGCGGAGAGTGGAATGG |
| 32.88 | 91 | R: ACGTGGGCGTGAGTTCTTTG | |||
| 0.95 | |||||
| PPIA | NM_021130 | -4.81 | 51% | 7 | F: GCTTTGGGTCCAGGAATGG |
| 21.52 | 59 | R: TTGTCCACAGTCAGCAATGG | |||
| 0.96 |