Figure 6.

Hyperoxia exposure induces pulmonary vascular remodeling.

Notes: Representative α-SMA-stained resistance pulmonary arteries obtained at 14 days of age from neonatal mice exposed to air (A) or hyperoxia (B). Quantitative analysis of pulmonary vascular remodeling by medial thickness index (C). Representative immunoblot showing α-SMA protein expression (D). Densitometric analyses wherein the α-SMA band intensities were quantified and normalized to β-actin (E). Values are mean ± SD from three individual animals in each group from one experiment. Significant differences between air and hyperoxia groups are indicated by *P<0.05. Scale bar =100 µM.

Abbreviations: WT, wild type; α-SMA, alpha smooth muscle actin; SD, standard deviation.