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. 2016 Jul 19;11(7):e0159552. doi: 10.1371/journal.pone.0159552

Table 1. qPCR conditions.

Primer sequences, NCBI accession numbers and annealing temperatures used in the qPCR analysis.

Gene name Accession number Primer sequences Annealing temp.
HPRT NM_013556 F: 5’-GCAGTACAGCCCCAAAATGG-3’ 65
R: 5’-AACAAAGTCTGGCCTGTATCCAA-3’
Cyclophilin NM_011149 F: 5’-GAGACTTCACCAGGGG-3’ 65
R: 5’-CTGTCTGTCTTGGTGCTCTCC-3’
Ubiquitin NM_019639 F: 5’-AGCCCAGTGTTACCACCAAG-3’ 65
R: 5’-CTAAGACACCTCCCCCATCA-3’
CAR (Nr1i3) NM_009803.5 F: 5’-CGCAGTCCATGCAGGGTTCCA-3’ 65
R: 5’-ACTCCGGGTCTGTCAGGGGA-3’
PXR (Nr1i2) NM_010936.3 F: 5’-ACCCTAATGGTGGCTTCCAG-3’ 65
R: 5’-GGTTTGCTGGGCGTTGATG-3’
Cyp2b10 NM_009999.4 F: 5’-GGACAGTTGCTGTCGTTGAGCCA-3’ 65
R: 5’-GCAGGCGCAAGAACTGACGG-3’
Cyp1a2 NM_009993 F: 5’-CAGGAGCACTACCAAGACTTCA-3’ 65
R: 5’-TGGATCTTCCTCTGCACGTT-5’
Cyp2d22 NM_001163472.1 F: 5’-CACCGGTAAAGGTAGCTGGAGT-3’ 65
R: 5’-CCAGCTGTAGGCTGAACAGG-3’
Cyp3a11 NM_007818 F: 5’-AACCTGGGTGCTCCTAGCAA-3’ 65
R: 5’-AGCAAGGAGAGGCGTTTGAC-3’
Cyp2c65 NM_028191.2 F: 5’-CAACCCAGAGAAGTTTGACCCCA -3’ 65
R: 5’-CTCTCCCACACAAATCCGTTTTC-3’
Cyp2c37 NM_010001 F: 5’-GATGGCAATCAACCATTGCAAAA-3’ 65
R: 5’-CTTGCCGATCACATGCTCAA-3’
Cyp2c38 NM_010002 F: 5’-GGTAGAGAAAACATCCCAATGTCTG-3’ 65
R: 5’-GACATTGCATGGAGCACAGC-3’