Figure 2.

Retraction of adhesive structures in OCs induced by OPG. (A) OPG-induced adhesive region detachment in OC examined by SEM. OCs were cultured on glass coverslips with (c-f) or without (a, b) collagen-I treatment. Various concentrations of OPG (0 (b, c), 20 (d), 40 (e) and 80 (f) ng/mL) were added for 24 h. White arrows indicate lamellipodia; white arrowheads indicate filopodia. (B) Dynamic monitoring of OCs treated with OPG using impedance technology. Cell index (CI) values were normalized to the time point of administration. Black arrow: OPG addition. Representative data are averaged from 3 wells. All experiments were repeated at least 3 times.