Figure 5. Local and systemic inflammation in Δ/Δep2^TX mice.

(A) PCR analysis of tail tissue (left) and immunoblot analysis of epidermal lysates obtained from Δ/Δep2^TX animals. (B) Macroscopic appearance of Δ/Δep2^TX mice and histological analysis of H&E sections. Scale bars, 25 µm. Infiltrating cells: mast cells (MC; TB⁺), activated mast cells (β1 Tryptase⁺; modest), granulocytes (GR; esterase⁺). The plot shows a quantification of the histological analysis. (C) Mild splenomegaly with increased numbers of Mac1⁺Gr1⁺ cells in Δ/Δep2^TX animals. (D) Activated T cells, B cells and dendritic cells in the lymph nodes of Δ/Δep2^TX animals. (E) Mild lymphocytosis and significantly elevated granulocyte numbers in Δ/Δep2^TX mice. The right panel shows comparable IgE plasma levels in control and Δ/Δep2^TX animals. Data are plotted as mean ± SEM (n = 5; p1 = 0.034, p2 = 0.014, p3 = 0.005, p4 = 2.63E-4, p5 = 0.001, p6 = 0.001, p7 = 0.019 and p8 = 0.042).

DOI: http://dx.doi.org/10.7554/eLife.14012.010

Figure 5—figure supplement 1. Representative FACS analysis of lymph node and spleen cells isolated from adult F/F2^TX Δ/Δep2^TX animals.

The percentage of activated lymphocytes in lymph nodes was assessed by combining lineage specific markers (CD4 and CD8 for T lymphocytes; B220 for B lymphocytes) with the activation marker CD69. Activated dendriditic cells were identified by staining with CD11c and MHC II antibodies. Myeloid cells in the spleen were analyzed by staining with Mac1 and Gr1 antibodies. The percentage of single and double positive cells is indicated.

DOI: http://dx.doi.org/10.7554/eLife.14012.011