Figure 2. 2p-RAM optics and optomechanics.
(A) Optical layout. Several routing mirrors were omitted for clarity. See text for detailed description. (B) Power transmission measurements at 970 nm. (C) CAD model of the microscope. The microscope was assembled on a vertically mounted breadboard measuring 1.0 m x 0.5 m. The objective is 63 mm wide, 120 mm long, and weighs 1.43 kg. (D) Photograph of microscope objective. (D, E, photocredit; Matt Staley). (E) Photograph of vertically mounted breadboard.
Figure 2—figure supplement 1. Pulse width measurements.
We measured the pulse width (full-width-at-half-max) using an autocorrelator. The autocorrelator was placed after the first telescope and EOM. For a Gaussian laser pulse, the width of the autocorrelation is 1.41 times the pulse width of the individual laser pulse. (A) Autocorrelation at the autocorrelator without GDD compensation. (B) Autocorrelation at the autocorrelator with GDD compensation. The pulse is now very broad due to the pre-chirp. (C) The autocorrelation at the specimen. As a sample we used a uniform fluorescent solution (Sigma F6377, 100 µM, 1 mm thick) and recorded fluorescence using the PMT.
Figure 2—figure supplement 2. Distances between the critical 2p-RAM components.
(A) CAD model of the microscope with critical distances for assembling the 2p-RAM. PR: pupil relay, RFO: remote focus objective, IO: immersion objective, res.: resonant scan mirror.