Figure 3. Transcriptional memory leads to remodeling of COMPASS.
(A) ChIP against COMPASS subunits Swd1-GFP, Bre2-GFP, Sdc1-GFP, and Spp1-GFP from cells grown under repressing, activating or memory conditions. (B and C) ChIP against Spp1-GFP at the indicated times either after shifting cells from activating to repressing conditions (B) or after shifting cells back from repressing to activating conditions following 3 hr of repression (C). All ChIP experiments are averages of three biological replicates ± standard error of the mean, quantified relative to input using primers to amplify the INO1 promoter (−348 to −260) or the PRM1 CDS. *p<0.05, compared with the repressing condition (A) or compared with the 0 min time point (B and C) (Student’s t-test).