Figure 5. Molecular requirements for PIC assembly during transcriptional memory.
(A, D, G and J) Confocal micrographs of the indicated proteins fused to FRB-GFP before or after treatment with rapamycin for 90 min. (B, E, H and K) ChIP against RNAPII from strains expressing Spt15-FRB-GFP (B), Med1-FRB-GFP (E), TFB1-FRB-GFP (H) or Kin28-FRB-GFP (K), grown under either activating or memory conditions, before or after treatment 1 µg/ml of rapamycin. (C, F, I and L) ChIP against H3K4me2 from strains expressing Spt15-FRB-GFP (C), Med1-FRB-GFP (F), TFB1-FRB-GFP (I) or Kin28-FRB-GFP (L), grown under either activating or memory conditions, before or after treatment 1 µg/ml of rapamycin. All ChIP experiments are averages of three biological replicates ± standard error of the mean, quantified as in panel 1A, using primers to amplify the INO1 promoter (−348 to −260) or the PRM1 CDS. Mock treatment had no effect (not shown). *p<0.05, compared with 0 min rapamycin (Student’s t-test).