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. Author manuscript; available in PMC: 2016 Jul 19.
Published in final edited form as: Hum Pathol. 2015 Apr 15;46(7):948–956. doi: 10.1016/j.humpath.2015.03.017

Figure 4.

Figure 4

PAX8 expression does not induce neoplastic transformation in OSECs. (A) PAX8 expression is increased in normal, TERT immortalized OSECs following CMYC overexpression. (* P = 0.003) (B) Data from the ENCODE project shows five CMYC binding sites (demonstrated by ChIPseq, red boxes) located upstream of the PAX8 promoter, including four that lie within common enhancer regions (dashed boxes). (C-F) IOE19CMYC cells stably expressing GFP or a PAX8-GFP fusion protein were assayed for cellular transformation. (C) IOE19CMYC.PAX8 cells express higher levels of PAX8 than the parental cells (IOE19CMYC), IOE19CMYC.GFP control cells), and HGSOC cells (OVCA433)(P < 0.0001, unpaired T-test). (D) IOE19CMYC.PAX8 cells were less migratory than GFP control cells in an in vitro transwell migration assay. (* P = 0.028) (E) Cellular invasion was not significantly different in IOE19CMYC.GFP and IOE19CMYC.PAX8 cells in IOE19CMYC.GFP vitro. (F) Anchorage independent growth was not significantly different between and IOE19CMYC.PAX8 cells using a soft agar assay. OSECs = ovarian surface epithelial cells, IOE19 = immortalized ovarian surface epithelial cells.