Figure 2. Stability of AFP_158-166 peptide-HLA-A*02:01 complex, CD80, CD86, and IL-15 expression in BA15 cells after γ-ray irradiation.

A. FCM revealed that the expression of HLA-A2, CD80, and CD86 were not significantly affected by different dosages of irradiation. B. ELISA showed that the secretion of IL-15 in BA15 cells decreased after exposure to 30 Gy of irradiation but was stable at lower dosages. C. HPLC showed that the eluting peak corresponding to the synthetic AFP_158-166 peptide was found in acid-stripped BA15 cells both pre- and post-irradiation. Mass spectrometry revealed that the molecular weight of the peptide in this eluting peak was the same as that of the synthetic peptide. Error bars indicate standard deviations.