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. 2016 Feb 23;7(14):17957–17969. doi: 10.18632/oncotarget.7647

Figure 5. DACT2 inhibits Wnt signaling in esophageal cancer cell lines and a xenograft mouse model.

Figure 5

(A) The expression levels of DACT2, active-β-catenin, p-β-catenin, total β-catenin, c-Myc, p-CDC2 (Y15), CDC2 and cyclin B1 were detected by western blot in DACT2 unexpressed and re-expressed KYSE150 and KYSE450 cells. The results were validated by knocking down DACT2 in YES2 cells. (B) Representative results of DACT2 re-expressed and unexpressed KYSE150 cell xenograft tumors in mice. (C) The tumor growth curve of DACT2 re-expressed and unexpressed KYSE150 cells. *P < 0.05. (D) The average weight of DACT2 re-expressed and unexpressed tumors. *P < 0.05. (E) The expression of DACT2 (upper panels) and p-β-catenin (lower panels) in re-expressed and unexpressed KYSE150 cell xenografts by IHC. Magnification: ×400.