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. 2016 Feb 22;7(14):17970–17985. doi: 10.18632/oncotarget.7602

Figure 5. Rac1b upregulates and activates the JNK/c-JUN/cyclin-D1 and AKT2/MCL1 pathways in SW480 cells.

Figure 5

Two stable SW480 cell-lines, transfected with LV-puro or LV-Rac1b, were cultured in 10% and 0% serum, and 0% serum supplemented with a JNK (10 mM, 48 h; A) or ATK2 (10 mM, 48 h; B) inhibitor. A. and B. Changes in JNK, p-JNK, c-JUN, p-c-JUN, cyclin-D1, AKT2, p-AKT, MCL1 and PARP-25 were compared by western blotting. C. Densities in A and B were first normalized to GAPDH and then to LV-puro cells. N= 4-5. *p < 0.05 vs. LV-puro cells in each corresponding serum condition.