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. 2016 Feb 25;7(14):18021–18035. doi: 10.18632/oncotarget.7694

Figure 1. Overexpression of PLA2G16 increases osteosarcoma cell proliferation, clonogenicity and anchorage-independent colony formation.

Figure 1

(A) PLA2G16 expression levels in osteosarcoma cell lines as determined by qRT-PCR (upper) and western blotting analyses (lower). (B) PLA2G16 or PLA2G16-C113S overexpression levels in Saos2 and MG63 cells as determined by qRT-PCR (upper) and western blot (lower) analyses. MTT analysis (C), Low density colony formation (D) and soft agar assays (E) were performed with PLA2G16 or PLA2G16-C113S overexpression cells. Results are shown as the mean ± SD of three independent experiments and one way ANOVA was used to determine the statistical significance between PLA2G16 overexpression (WT) and empty vector pBABE-puro (CTL) cells. ** indicates p < 0.01; *** indicates p < 0.001; CTL, pBABE-puro; WT, pBABE-FLAG-PLA2G16; MUT, pBABE-FLAG-PLA2G16-C113S.