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. 2016 Feb 25;7(14):18021–18035. doi: 10.18632/oncotarget.7694

Figure 3. Knockdown of PLA2G16 decreases osteosarcoma cell proliferation, clonogenicity, anchorage-independent colony formation, migration and invasion.

Figure 3

(A) PLA2G16 expression in HOS cells with shRNA knockdown was determined by qRT-PCR (upper) and western blot (lower) analyses. MTT analyses (B), low density colony formation (C), Anchorage-independent growth (D), wound-healing (E) and Matrigel transwell (F) assays were performed in HOS cells with PLA2G16 knockdown. The colonies were counted from low density colony formation and soft agar assays after incubation and staining. Results are shown as the mean ± SD of three independent experiments and One way ANOVA was used for statistical analyses to compare sh1 or sh2 knockdown to control cells ** indicates p < 0.01; *** indicates p < 0.001; sh1 and sh2 were shRNAs against PLA2G16.