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. 2016 Feb 23;7(14):18050–18064. doi: 10.18632/oncotarget.7633

Figure 4. ROS generation is the key regulator of EF24-induced apoptosis.

Figure 4

A. Intracellular ROS generation induced by EF24 was measured in SGC-7901 cells by staining with DCFH-DA (10 μM), DAF-FM-DA (5 μM) or DHE (5 μM) and flow cytometry analysis. B. Intracellular ROS generation induced by increasing doses of EF24 was measured in SGC-7901 cells by flow cytometry. C.-D. SGC-7901 cells were pre-incubated with NAC or catalase for 2 h before exposure to EF24 (7.5 μM) for 1 h. Intracellular ROS generation was measured by flow cytometry. E.-F. Blocking of ROS generation abolished the cytotoxicity of EF24. SGC-7901 cells were pre-incubated with or without NAC or catalase for 2 h before exposure to EF24 (7.5 μM). Cell cycle arrest and percentage of cell apoptosis were determined by flow cytometry.