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. 2016 Feb 27;7(14):18146–18158. doi: 10.18632/oncotarget.7776

Figure 2. Expression and secretion of TRPV1, SP, and CGRP in DRG.

Figure 2

(A) The graph shows the mRNA expression of TRPV1, SP, and CGRP in DRG in different culture systems. The expression levels in co-culture of AsPc-1 cells with PSCs were higher than in other groups (p < 0.05) or in DRG cultured alone as a control. (B) The protein expression of TRPV1, SP, and CGRP in DRG in different culture systems determined by Western blotting. The protein results were similar to mRNA; DRG were cultured alone as a control. (C) Co-culture of AsPc-1 cells and PSCs increased the secretion of pain factors, SP, and CGRP by ELISA, compared with other groups (p < 0.05).