Skip to main content
. 2016 Feb 22;7(14):18247–18261. doi: 10.18632/oncotarget.7570

Figure 6. miR-206 inhibits c-Met overexpression-induced activation of Akt/mTOR pathway.

Figure 6

A, B. A549 cells (A) and 95D cells (B) were transfected with c-Met overexpression plasmid (ex-Met) or control (ex-NC). 48 h after transfection, the cells were collected. The expression of p-c-Met, c-Met, p-Akt, Akt, p-mTOR, mTOR was analyzed by western blot assay. The results showed that the expression levels of these proteins were increased significantly (except Akt in A549 cells). Cotransfected with miR-206 mimics and c-Met overexpression plasmid significantly decreased the expression of p-Met, Met, p-Akt, Akt, p-mTOR. Relative protein expression was calculated by gray value of indicated protein/GAPDH. ** P<0.01 vs its control as indicated in Figure.