Figure 6. Inhibition of MTA1 and its associated signaling by pterostilbene (PTER) in Pten^f/f mice.

(A) Immunoblots of MTA1, p-Akt, Akt, AR, IL-1β, Hsp90 and E-cadherin (E-cad) in the prostate tissues of vehicle and PTER treated Pten^f/f mice, isolated at indicated ages. Hsp70 was a loading control. (B) Comparative analysis of MTA1 binding in the prostate tissues of Pten^+/f mice on Ctrl- and PTER-Diet. Representative MTA1 ChIP-Seq tracks for IL-1β, E-cadherin and Vimentin gene loci at 10 kb resolution are shown. (C) Comparison of IHC staining of MTA1, p-Akt and AR in the prostate sections with carcinoma lesions from representative 10-, 25- and 33-week old vehicle or PTER treated Pten^f/f mice and NP controls. Scale bars, 100 μm. (D) Immunoblots of MTA1, p-Akt, Akt and AR in the dissected prostate lobes from vehicle or PTER-treated 10-, 15-, 20- and 25-week-old Pten^f/f mice. Hsp70 was used as a loading control. For quantitation of MTA1, p-Akt/Akt and AR expression in prostate lobes of Pten^f/f mice at different ages (n = 3/group) see Supplementary Figure S5.