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. 2016 Feb 23;7(14):18620–18630. doi: 10.18632/oncotarget.7620

Figure 5. Nek7 gene silencing suppressed growth of SMMC7721 xenograft tumors.

Figure 5

A. Tumors isolated from mice of different groups are shown. B. Growth curves of xenograft tumors from the experiments with BALB/c nude mice. Changes in tumor volumes measured at the indicated days are shown. C. Therapeutic lenti-shNek7-1 reduced the tumor weight. Real-time PCR and Western blot assays to determine the mRNA and protein levels of cell growth associated genes, Bcl-2, cyclinD1 and cyclinB1 in HCC cells D. and dissected tumors E, F. Immunohistochemical staining was used to detect the Nek7 and Ki-67 expression pattern in xenograft tumors from different groups. β-actin was used as internal control for Western blot and Real-time PCR. Experiments were repeated three times. (*p < 0.05).