Figure 3. PARP6 inhibits tumor formation in vivo.

A. Empty vector and FL-PARP6 transfectant cells were subcutaneously inoculated into the lower right flank of the nude mice. B. Representative images of empty vector and FL-PARP6 transfectant tumors following subcutaneous injection. C. The tumor volume was calculated using the following formula: tumor volume (mm³) =0.5 × length (mm) × width² (mm²). The tumor weight was measured. Left graph shows tumor volume, and right graph shows tumor weight in empty vector and FL-PARP6 transfectant tumors. **P < 0.01. D. Expression of PARP6 protein in empty vector and FL-PARP6 transfectant tumors was examined by immunohistochemistry.