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SIRT2 levels were modified in A549 cells by transfection the cells with either SIRT2, or shSIRT2 or a scrambled sequence as a control (scr). A. RT-qPCR for SIRT2 mRNA in SIRT2-modified A549 cells. B. Representative Western blots for SIRT2, Sep2 and p27 in SIRT2-modified A549 cells. C–E. Quantification for SIRT2 (C), Sep2 (D) and p27 (E) protein in SIRT2-modified A549 cells. *p<0.05. N=5.
