Figure 6. SIRT2 decreases Skp2 levels through induction of Skp2 deacetylation.

The SIRT2-overexpressing A549 cells were exposed with TSA, an effective inhibitor for the class I and II HDACs, including SIRT2. A. The levels of SIRT2, Skp2 and p27 in SIRT2-depleted A549 cells with/with TSA were analyzed by Western blot, shown by representative Western blot. B–D. Quantification for SIRT2 (B), Sep2 (C) and p27 (D) protein in SIRT2-modified A549 cells. *p<0.05. NS: non-significant. N=5.