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. 2016 Mar 8;7(14):19018–19030. doi: 10.18632/oncotarget.7968

Figure 4. ZLM-7 inhibited HUVECs proliferation.

Figure 4

A. Cell proliferation was determined by MTT assay after treatment with ZLM-7 or CA-4 at varying times (12, 24, 48 and 72 h). B. Cell proliferation was determined by MTT assay after treatment with (left) ZLM-7 or (right) CA-4 for 48 h in the presence of VEGF. C. Representative images and quantitative data of the inhibitory effects of ZLM-7 and CA-4 in HUVEC clonogenic survival assay. D. Toxicity examination using LDH cytotoxicity assay of HUVECs after ZLM-7 or CA-4 treatment for 48 h. E. Annexin V-FITC analysis of apoptosis in HUVECs after treatment with ZLM-7 or CA-4 for 48 h by FACS. All data were represented as means ± SD, n = 3, * P < 0.05 compared to control.