Table 2.

DPPH radical scavenging activity, reducing power and phenolics content of acetone extracts of lichens Melanelia subaurifera and Melanelia fuliginosa, and their major metabolites

Tested lichen species and compounds	DPPH radical scavenging IC50 (μg/ml)
Melanelia subaurifera	165.13 ± 1.78
Melanelia fuliginosa	266.32 ± 2.08
Lecanoric acid	424.51 ± 1.42
2′-O-methyl anziaic acid	121.52 ± 1.12
Ascorbic acida	6.42 ± 0.18

Tested lichen species and compounds	Reducing power Absorbance (700 nm)
	1000 µg/ml	500 µg/ml	250 µg/ml	125 µg/ml
Melanelia subaurifera	0.1189 ± .031	0.0744 ± .004	0.0327 ± .001	0.0316 ± .003
Melanelia fuliginosa	0.0905 ± .006	0.0623 ± .003	0.0468 ± .002	0.0252 ± .002
Lecanoric acid	0.0336 ± .001	0.0178 ± .002	0.0170 ± .001	0.0165 ± .001
2′-O-methyl anziaic acid	0.2048 ± .042	0.0335 ± .002	0.0128 ± .002	0.0056 ± .000
Ascorbic acida	2.113 ± .032	1.654 ± .021	0.0957 ± .008	0.0478 ± .004

Lichen species	Phenolics content (μg PE/mg of extract)
Melanelia subaurifera	41.33 ± 1.065
Melanelia fuliginosa	39.68 ± 1.013

Values are expressed as mean ± SD of three parallel measurements

PE pyrocatechol equivalents

^aAscorbic acid was used as standard